Indian Oil has patented a method to enhance biomass and omega-3 fatty acid production in Thraustochytrid microalgae using a specific chemical mixture. The invention includes a novel strain, Schizochytrium sp. (MTCC 5980), and a media supplement composition featuring citric acid, malic acid, and benzyl adenine purines. GlobalData’s report on Indian Oil gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Indian Oil, Bioethanol production GMOs was a key innovation area identified from patents. Indian Oil's grant share as of July 2024 was 60%. Grant share is based on the ratio of number of grants to total number of patents.
Method for enhancing omega-3 fatty acids in microalgae
The granted patent US12065625B2 outlines a media supplement composition designed to enhance the production of omega-3 fatty acids from a carbon substrate. The composition consists of a mixture of modulators, specifically including citric acid (0.25-0.65%), malic acid (0.25-1%), and benzyl adenine purines (0.0005-0.0015%). The patent further details variations in the concentration of these components, with specific ranges for citric acid (0.1-10 g/L), malic acid (0.1-12 g/L), and benzyl adenine purines (1-15 mg/L). Additionally, the composition can be formulated in a specific ratio of 0.4:0.58:0.002 for citric acid, malic acid, and benzyl adenine purines, respectively.
The patent also describes a method for utilizing this media supplement composition to enhance omega-3 fatty acid production. This method involves adding the composition to a growth medium containing a carbon substrate, such as glycerol, and culturing the microorganism Schizochytrium sp. (MTCC 5980) in the supplemented medium. The method can be performed without nitrogen stress induction and is characterized by specific conditions, including a pH range of 5.3-9.5 and a culturing temperature of 30-35°C for 24 hours. The process is designed to be conducted in a single continuous reactor, optimizing the production of omega-3 fatty acids from the specified carbon substrate.
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